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  • Featured Product
  • KD/KO Validated

SMN Polyclonal antibody

SMN Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22329-1-AP

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    WB result of SMN antibody (22329-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HEK-293 cells.

  • Western Blot (WB) analysis of K-562 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of K-562 using 22329-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HepG2 using 22329-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of Jurkat using 22329-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    IF Staining of HepG2 using 22329-1-AP

    Immunofluorescent analysis of HepG2 cells using 22329-1-AP (SMN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22329-1-AP is SMN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMN fusion protein Ag17798
Full Name survival of motor neuron 2, centromeric
Calculated Molecular Weight 282 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000908
Gene Symbol SMN2
Gene ID (NCBI) 6607
RRIDAB_2879074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Protocols

Product Specific Protocols
WB protocol for SMN antibody 22329-1-APDownload protocol
IHC protocol for SMN antibody 22329-1-APDownload protocol
IF protocol for SMN antibody 22329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols