SMPDL3B Polyclonal antibody

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  WB analysis using 16552-1-AP

  COLO 320 cells lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SMPDL3B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16552-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

ASM like phosphodiesterase 3b, ASML3B, SMPDL3B

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16552-1-AP targets SMPDL3B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9535

  Product name: Recombinant human SMPDL3B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-336 aa of BC014444

  Sequence: MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWALINSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKNQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADPGQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKV

• Full Name: sphingomyelin phosphodiesterase, acid-like 3B
• Calculated Molecular Weight: 373aa,42 kDa; 455aa,51 kDa
• Observed Molecular Weight: 42 kDa, 50-65 kDa
• GenBank Accession Number: BC014444
• Gene Symbol: SMPDL3B
• Gene ID (NCBI): 27293
• RRID: AB_2878276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92485
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Mol Biosci; SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia; Authors - Huiqing Qu ; KO Validated
rat	WB,IHC	Basic Clin Pharmacol Toxicol; PMID: 29923306; Authors - Xin Li
mouse	WB	JHEP Rep; Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease.; Authors - Taesik Gwag
human,pig,monkey	WB,IF	iScience; Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism; Authors - Huan-Huan Shen ; KD Validated
Human		Turk J Med Sci; SMPDL3B contributes to gastric adenocarcinoma cells progression by promoting the infiltration of M2 macrophages; Authors - Li Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody works well for mouse liver tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue