Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16552-1-AP targets SMPDL3B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9535 Product name: Recombinant human SMPDL3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-336 aa of BC014444 Sequence: MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWALINSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKNQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADPGQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKV Predict reactive species |
| Full Name | sphingomyelin phosphodiesterase, acid-like 3B |
| Calculated Molecular Weight | 373aa,42 kDa; 455aa,51 kDa |
| Observed Molecular Weight | 42 kDa, 50-65 kDa |
| GenBank Accession Number | BC014444 |
| Gene Symbol | SMPDL3B |
| Gene ID (NCBI) | 27293 |
| RRID | AB_2878276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92485 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMPDL3B (sphingomyelin phosphodiesterase acid-like 3B) is lipid-modulating phosphodiesterase, which is active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
| WB protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SMPDL3B antibody (Cat# 16552-1-AP) has 7 citations published in Immunity, J Transl Med, JHEP Rep, iScience, Front Mol Biosci, Basic Clin Pharmacol Toxicol, and Turk J Med Sci. Research fields include viral infection and cGAS-STING signaling, myalgic encephalomyelitis, obesity-associated NAFLD, viral replication and lipid metabolism, acute myeloid leukemia, vascular insulin resistance in diabetes, and gastric adenocarcinoma progression.
| Species | Application | Title |
|---|---|---|
Immunity Membrane integrity changes upon viral infection activate sphingomyelinase SMPDL3B to restrict cGAS-STING signaling via cGAMP degradation | ||
J Transl Med SMPDL3B a novel biomarker and therapeutic target in myalgic encephalomyelitis | ||
JHEP Rep Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease. | ||
iScience Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism
| ||
Front Mol Biosci SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia
| ||
Reviews
What customers say
The single review for this antibody is highly positive, with a 5-star rating. The reviewer used it for Western Blot at a 1:10,000 dilution on mouse liver tissue and reported that it works well for this application. Overall, the feedback indicates reliable performance in detecting the target protein in mouse liver samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (02-15-2024) | This antibody works well for mouse liver tissues.
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