SNRPD1 Polyclonal antibody

SNRPD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10352-1-AP

Synonyms

HsT2456, Sm D autoantigen, Sm D1, SMD1, snRNP core protein D1, SNRPD, SNRPD1

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 10352-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10352-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10352-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

10352-1-AP targets SNRPD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPD1 fusion protein Ag0364
• Full Name: small nuclear ribonucleoprotein D1 polypeptide 16kDa
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC001721
• Gene Symbol: SNRPD1
• Gene ID (NCBI): 6632
• RRID: AB_2193875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD1 is one Sm components of the small nuclear ribonucleoprotein complexes(snRNPs). It's a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. This antibody detectes 13-16 kDa (SMD1) and 30 kDa (SMD1/SMD2 dimer form; PMID:15525645).

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
mouse	IHC,WB	Pharmacol Res; Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses.; Authors - Jing Wang
mouse	WB	J Proteome Res; Signaling in insulin-secreting MIN6 pseudoislets and monolayer cells.; Authors - Chowdhury Azazul Islam AI
human	WB	Neuron; Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes; Authors - Norah Al-Azzam