SOD2 Polyclonal antibody

SOD2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24127-1-AP

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, DU 145 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24127-1-AP is SOD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, goat, human, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD2 fusion protein Ag21388
Full Name superoxide dismutase 2, mitochondrial
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a 

mitochondrial enzyme that dismutates superoxide ions.


What is the molecular weight of SOD2? Is SOD2 post-translationally modified?

The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be 

post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2 

increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068), 

while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).


What is the subcellular localization of superoxide dismutases?

SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is 

predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and 

nucleus (PMID: 11507097), while SOD3 localizes extracellularly.


What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?

Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial 

matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals 

to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses 

they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases 

and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from 

oxidative damage (PMID: 19228881).


What is the tissue expression pattern of SOD2?

SOD2 is ubiquitously expressed.


Can SOD2 be used as a marker of oxidative stress?

SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2 

activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress 

because its expression is not always altered by oxygen stress. 


Protocols

Product Specific Protocols
WB protocol for SOD2 antibody 24127-1-APDownload protocol
IHC protocol for SOD2 antibody 24127-1-APDownload protocol
IF protocol for SOD2 antibody 24127-1-APDownload protocol
IP protocol for SOD2 antibody 24127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Redox Biol

Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.

Authors - Xiaopei Wu
mouseWB

Am J Transl Res

Effects of autophagy on the spermatogonia of chronic hypoxic mice.

Authors - Shaoming Zhu
mouseWB

Molecules

Sweroside Alleviated LPS-Induced Inflammation via SIRT1 Mediating NF-κB and FOXO1 Signaling Pathways in RAW264.7 Cells.

Authors - Rui Wang
mouseWB

J Neurosci Res

Nicotinamide mononucleotide alters mitochondrial dynamics by SIRT3-dependent mechanism in male mice.

Authors - Nina Klimova
mouseWB

Theranostics

p66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species.

Authors - Yan Zhao
ratWB

Environ Toxicol Pharmacol

Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.

Authors - Xiang Yan