|Positive WB detected in||DU 145 cells, HeLa cells, mouse brain tissue, rat brain tissue, MCF-7 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24127-1-AP is SOD2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, goat, human, mouse, pig, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SOD2 fusion protein Ag21388|
|Full Name||superoxide dismutase 2, mitochondrial|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC016934|
|Gene ID (NCBI)||6648|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a
mitochondrial enzyme that dismutates superoxide ions.
What is the molecular weight of SOD2? Is SOD2 post-translationally modified?
The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be
post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2
increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068),
while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).
What is the subcellular localization of superoxide dismutases?
SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is
predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and
nucleus (PMID: 11507097), while SOD3 localizes extracellularly.
What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?
Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial
matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals
to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses
they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases
and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from
oxidative damage (PMID: 19228881).
What is the tissue expression pattern of SOD2?
SOD2 is ubiquitously expressed.
Can SOD2 be used as a marker of oxidative stress?
SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2
activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress
because its expression is not always altered by oxygen stress.
|Product Specific Protocols|
|WB protocol for SOD2 antibody 24127-1-AP||Download protocol|
|IHC protocol for SOD2 antibody 24127-1-AP||Download protocol|
|IF protocol for SOD2 antibody 24127-1-AP||Download protocol|
|IP protocol for SOD2 antibody 24127-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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