SOX17 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOX17 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 24903-1-AP

Synonyms

SOX17, Transcription factor SOX 17

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Validation Data Gallery

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  WB analysis using 24903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 24903-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C6 cells, PC-3 cells, mouse embryo

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 11 publications below

Product Information

24903-1-AP targets SOX17 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX17 fusion protein Ag19157
• Full Name: SRY (sex determining region Y)-box 17
• Calculated Molecular Weight: 414 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC111365
• Gene Symbol: SOX17
• Gene ID (NCBI): 64321
• RRID: AB_2879789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor SOX 17 (SOX17) also named as SRY box 17 is a 414 amino acid protein, which contains one Sox C-terminal domain and one HMG box DNA-binding domain. SOX17 as a transcription regulator binds target promoter DNA and bend the DNA via WNT3A. SOX17 is a marker of endodermal cells and a transcriptional regulator containing a DNA binding domain called the HMG box. In mouse embryos, SOX17 plays critical roles in the growth and differentiation of definitive endodermal cells (PMID: 11973269), the remodeling of endothelial cells (PMID: 16895970), hindgut endoderm expansion with localization of primordial germ cells (PMID: 19371732), and gallbladder/bile duct formation (PMID: 19913509).

Publications

Species	Application	Title
human	IF	Mater Today Bio; Nanofiber-microwell cell culture system for spatially patterned differentiation of pluripotent stem cells in 3D; Authors - Youyi Tai
mouse	WB	Stem Cell Reports; Phosphorylation of Threonine343 Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency.; Authors - Xianmixinuer Abulaiti
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun
human	IF	Stem Cell Res; Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA).; Authors - Jiajia Yang
human	IF	Stem Cell Res; Generation of a Friedreich's Ataxia patient-derived iPSC line USFi001-A.; Authors - Mariana Burgos Angulo
human	IF	Stem Cell Res; Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C).; Authors - Jiajia Yang