SPACA1 Polyclonal antibody

SPACA1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12829-1-AP

Synonyms

SAMP32, SPACA1, sperm acrosome associated 1

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Validation Data Gallery

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  IHC staining of human testis using 12829-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12829-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12829-1-AP targets SPACA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPACA1 fusion protein Ag3549
• Full Name: sperm acrosome associated 1
• Calculated Molecular Weight: 294 aa, 32 kDa
• GenBank Accession Number: BC029488
• Gene Symbol: SPACA1
• Gene ID (NCBI): 81833
• RRID: AB_2877886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPACA1, also named as SAMP32, is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. SPACA1 is regulated by phosphorylation.

Publications

Species	Application	Title
mouse	WB	Free Radic Res; Heterozygous SOD1 deficiency in mice with an NZW background causes male infertility and an aberrant immune phenotype; Authors - Takujiro Homma