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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SPAG4L Polyclonal antibody

SPAG4L Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17495

Synonyms

dJ726C3.1, SPAG4L, SUN5, TSARG4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®594-conjugated SPAG4L Polyclonal antibody (CL594-17495)

    FC experiment of PC-3 using CL594-17495

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SPAG4L (CL594-17495) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17495 targets SPAG4L in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG4L fusion protein Ag11629
Full Name sperm associated antigen 4-like
Calculated Molecular Weight 379 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC026118
Gene Symbol SPAG4L
Gene ID (NCBI) 140732
RRIDAB_2919843
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

Protocols

Product Specific Protocols
FC protocol for CL594 SPAG4L antibody CL594-17495Download protocol
Standard Protocols
Click here to view our Standard Protocols