SPI1 Monoclonal antibody

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  WB analysis of Daudi using 66618-2-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66618-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66618-2-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66618-2-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human tonsillitis using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens).

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  IHC staining of Human tonsillitis using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens).

• 

  IHC staining of Human tonsillitis using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens).

• 

  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPI1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66618-2-Ig

Clone No.2H3D3

Publications(7)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, RAW 264.7 cells, rat spleen tissue
• Positive IHC detected in: Human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

66618-2-Ig targets SPI1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: NM_003120
• Gene Symbol: SPI1
• Gene ID (NCBI): 6688
• ENSEMBL Gene ID: ENSG00000066336
• RRID: AB_2881978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P17947
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Immunother Cancer; SPI1+CD68+ macrophages as a biomarker for gastric cancer metastasis: a rationale for combined antiangiogenic and immunotherapy strategies; Authors - Guofei Deng
mouse	IF	Acta Pharmacol Sin; PU.1/Spi1 exacerbates ischemia-reperfusion induced acute kidney injury via upregulating Gata2 and promoting fibroblast activation; Authors - Chen Zong
human	WB	Pathol Res Pract; INHBA, transcriptionally activated by SPI1, facilitates gastric cancer progression by inducing macrophage recruitment and M2 polarization via activating the TGF-β signaling to increase CCL2; Authors - Fan Zhang
human	WB,IHC	Discov Oncol; SPI1 is a prognostic biomarker of immune infiltration and immunotherapy efficacy in clear cell renal cell carcinoma; Authors - Huayi Feng
human	WB	Cancers (Basel); ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors; Authors - Qiyi Yi
human	WB,IF,CoIP	Exp Cell Res; IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis; Authors - Yilin Chen