Filter: AllWBIHC

WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)
Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 66618-2-Ig (same clone as 66618-2-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of rat spleen using 66618-2-Ig (same clone as 66618-2-PBS)
rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
WB analysis of Raji using 66618-2-Ig (same clone as 66618-2-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

SPI1 Monoclonal antibody, PBS Only

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66618-2-PBS

Clone No.2H3D3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66618-2-PBS targets SPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	fusion protein Predict reactive species
Full Name	spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated Molecular Weight	31 kDa
Observed Molecular Weight	32-38 kDa
GenBank Accession Number	NM_003120
Gene Symbol	SPI1
Gene ID (NCBI)	6688
ENSEMBL Gene ID	ENSG00000066336
RRID	AB_2881978
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P17947
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.