SPINK1 Polyclonal antibody

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  IHC staining of human pancreas using 13477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13477-1-AP (SPINK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 13477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13477-1-AP (SPINK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13477-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13477-1-AP (SPINK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13477-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13477-1-AP (SPINK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPINK1 Polyclonal Antibody for IHC, ELISA

Cat No. 13477-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PCTT, PSTI, SPINK1, Spink3, TATI

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Tested Applications

• Positive IHC detected in: human pancreas tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13477-1-AP targets SPINK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPINK1 fusion protein Ag4279
• Full Name: serine peptidase inhibitor, Kazal type 1
• Calculated Molecular Weight: 79 aa, 9 kDa
• GenBank Accession Number: BC025790
• Gene Symbol: SPINK1
• Gene ID (NCBI): 6690
• RRID: AB_2877954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00995
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Transl Cancer Res; Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2; Authors - Churan Shen