SPO11 Polyclonal antibody

SPO11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15509-1-AP

Synonyms

Cancer/testis antigen 35, CT35, SPO11

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Validation Data Gallery

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  WB analysis of mouse testis using 15509-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15509-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 15509-1-AP is SPO11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPO11 fusion protein Ag4340
• Full Name: SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC033591
• Gene Symbol: SPO11
• Gene ID (NCBI): 23626
• RRID: AB_10639508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spo11 is a protein used in a complex along with Mre11 and Rad50 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Several transcript variants encoding different isoforms have been found for this gene.