SPRYD5 Polyclonal antibody

SPRYD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20802-1-AP

Synonyms

SPRY domain containing 5, SPRYD5, TRIM51

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Validation Data Gallery

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  WB analysis of Jurkat using 20802-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20802-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20802-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20802-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20802-1-AP targets SPRYD5 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRYD5 fusion protein Ag14723
• Full Name: SPRY domain containing 5
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC005014
• Gene Symbol: SPRYD5
• Gene ID (NCBI): 84767
• RRID: AB_2878743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.