Somatostatin (64-81aa) Polyclonal antibody

Somatostatin (64-81aa) Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17512-1-AP

Synonyms

SMST, somatostatin, Somatostatin (64-81aa), SST

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Validation Data Gallery

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  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP Somatostatin-Specific antibody (green) and 66198-1-Ig Insulin antibody (red) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse pancreas tissue, mouse brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human pancreas tissue, mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:6400-1:25600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 2 publications below

Product Information

17512-1-AP targets Somatostatin (64-81aa) in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: somatostatin
• Calculated molecular weight: 13 kDa
• GenBank accession number: NM_001048
• Gene symbol: SST
• Gene ID (NCBI): 6750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Publications

Species	Application	Title
rat	IF	Aging Dis; PMID: 31440379; Authors - Fei Han
	IF	Cell Death Dis; Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.; Authors - Xiaoyi Zhang