Tested Applications
| Positive IHC detected in | mouse pancreas tissue, human pancreas cancer tissue, mouse brain tissue, rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue, mouse brain tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:6400-1:25600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17512-1-AP targets Somatostatin (64-81aa) in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | somatostatin |
| Calculated Molecular Weight | 13 kDa |
| GenBank Accession Number | NM_001048 |
| Gene Symbol | Somatostatin |
| Gene ID (NCBI) | 6750 |
| RRID | AB_2195910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61278 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Somatostatin (64-81aa) antibody 17512-1-AP | Download protocol |
| IHC protocol for Somatostatin (64-81aa) antibody 17512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Somatostatin (64-81aa) Polyclonal antibody (Cat# 17512-1-AP) has 11 citations across journals including Adv Mater, PNAS, Mol Psychiatry, Cell Death Dis, MedComm, Carbohydr Polym, Aging Dis, J Clin Endocrinol Metab, Heliyon, Life Sci Alliance, and G3. Research fields span diabetes and β-cell biology, Alzheimer's disease, depression/stress response, pancreatic development, organoid culture, diabetic nephropathy, and neuroscience. Applications include IF and IHC in mouse, rat, and human samples.
| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
MedComm (2020) Loss of Beta-Cell Identity and Function as a Mechanism of Secondary Failure of Sulfonylurea Therapy in Diabetes. | ||
Carbohydr Polym A sulfated manno-glucuronan ameliorates β-cell dysfunction in type 2 diabetes by targeting ALDH1A3. | ||
Cell Death Dis Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis. | ||
Mol Psychiatry Somatostatin-expressing neurons in the zona incerta regulate chronic stress response and modulate depression-like behaviors. | ||
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user applied it at a 1:100 dilution for immunofluorescence on human brain cortex FFPE sections and reported that the somatostatin signal (green) clearly and nicely marked a population of neurons. Overall, the feedback indicates strong staining performance and reliable results in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (04-05-2024) | Somatostatin (green) marked nicely a population of my neurons in human brain FFPE cortex.
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