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STAT6 Polyclonal antibody

STAT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51073-1-AP

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C



Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman spleen tissue, mouse lung tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51073-1-AP targets STAT6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 94 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC075852
Gene symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Protocols

Product Specific Protocols
WB protocol for STAT6 antibody 51073-1-APDownload protocol
IHC protocol for STAT6 antibody 51073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Evid Based Complement Alternat Med

PMID: 29333181

Authors - Wenting Ji

J Cardiovasc Pharmacol

Molecular analysis of curcumin-induced polarization of murine RAW264.7 macrophages.

Authors - Fangyuan Chen
humanWB

BMC Microbiol

IRAK-M alters the polarity of macrophages to facilitate the survival of Mycobacterium tuberculosis.

Authors - Pei Shen
mouseWB

Eur J Pharmacol

Mechanism of Corilagin interference with IL-13/STAT6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model.

Authors - Peng Du
mouseWB

Neuroscience

IL-4 Switches Microglia/macrophage M1/M2 Polarization and Alleviates Neurological Damage by Modulating the JAK1/STAT6 Pathway Following ICH.

Authors - Yang He
humanWB

Onco Targets Ther

CTHRC1 in Ovarian Cancer Promotes M2-Like Polarization of Tumor-Associated Macrophages via Regulation of the STAT6 Signaling Pathway.

Authors - Yihan Bai