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STAT6 Antibody

STAT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51073-1-AP

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C



Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman spleen tissue, mouse lung tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51073-1-AP targets STAT6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 94 kDa
Observed molecular weight 100-110 kDa
GenBank accession number BC075852
Gene symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Protocols

Product Specific Protocols
WB protocol for STAT6 antibody 51073-1-APDownload protocol
IHC protocol for STAT6 antibody 51073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Evid Based Complement Alternat Med

Study on the Inhibitory Effects of Ephedra Aconite Asarum Decoction on LPS-Induced Dendritic Cells.

Authors - Wenting Ji

J Cardiovasc Pharmacol

Molecular analysis of curcumin-induced polarization of murine RAW264.7 macrophages.

Authors - Fangyuan Chen
humanWB

BMC Microbiol

IRAK-M alters the polarity of macrophages to facilitate the survival of Mycobacterium tuberculosis.

Authors - Pei Shen
mouseWB

Eur J Pharmacol

Mechanism of Corilagin interference with IL-13/STAT6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model.

Authors - Peng Du
mouseWB

Neuroscience

IL-4 Switches Microglia/macrophage M1/M2 Polarization and Alleviates Neurological Damage by Modulating the JAK1/STAT6 Pathway Following ICH.

Authors - Yang He
humanWB

Onco Targets Ther

CTHRC1 in Ovarian Cancer Promotes M2-Like Polarization of Tumor-Associated Macrophages via Regulation of the STAT6 Signaling Pathway.

Authors - Yihan Bai