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STT3A Antibody

STT3A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12034-1-AP

Synonyms

B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

12034-1-AP targets STT3A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STT3A fusion protein Ag2698
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Calculated molecular weight 705aa,81 kDa
Observed molecular weight 90-100 kDa
GenBank accession number BC020965
Gene symbol STT3A
Gene ID (NCBI) 3703
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STT3A, also named as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, is a 705 amino acid prtein, which belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.

Protocols

Product Specific Protocols
WB protocol for STT3A antibody 12034-1-APDownload protocol
IHC protocol for STT3A antibody 12034-1-APDownload protocol
IF protocol for STT3A antibody 12034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Authors - Yini Wang
humanWB

MBio

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

Authors - David L Lin
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks