Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12034-1-AP targets STT3A in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2698 Product name: Recombinant human STT3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 475-705 aa of BC020965 Sequence: STWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT Predict reactive species |
| Full Name | STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 705 aa, 81 kDa |
| Observed Molecular Weight | 65-100 kDa |
| GenBank Accession Number | BC020965 |
| Gene Symbol | STT3A |
| Gene ID (NCBI) | 3703 |
| RRID | AB_2877818 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46977 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STT3A, also named as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, is a 705 amino acid prtein, which belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.
Publications
What published studies show
The STT3A Polyclonal antibody (Cat# 12034-1-AP) has 31 citations spanning high-impact journals including Nature, Cell, Cancer Discovery, Nature Metabolism, Nature Chemical Biology, and Journal of Clinical Investigation. Research fields cover N-glycosylation mechanisms, oncology (breast, liver, nasopharyngeal, colorectal, and soft tissue sarcoma cancers), viral infections, Alzheimer's disease, metabolic dysfunction-associated steatohepatitis, and inflammatory signaling. Applications cited include WB, IHC, IF, IP, and CoIP.
| Species | Application | Title |
|---|---|---|
Nature Structural basis of regulated N-glycosylation at the secretory translocon.
| ||
Cell Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB
| ||
Cancer Discov Pharmacologic Suppression of B7-H4 Glycosylation Restores Antitumor Immunity in Immune-Cold Breast Cancers.
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Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
J Clin Invest Integrated screening identifies GPR31 as a key driver and druggable target for metabolic dysfunction-associated steatohepatitis |
Reviews
What customers say
Both reviewers gave this antibody a perfect 5-star rating for Western Blot. One user described it as a "good antibody for Western," while the other simply noted it "works well." Overall, customers report reliable performance with no issues mentioned across two different lots tested in 2023.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Parijat (Verified Customer) (09-20-2023) | Good antibody for Western.
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Sarah-Eve (Verified Customer) (06-02-2023) | Works well
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