STXBP5 Polyclonal antibody

STXBP5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24512-1-AP

Synonyms

Tomosyn-1, Syntaxin-binding protein 5, LLGL3, Lethal(2) giant larvae protein homolog 3

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Validation Data Gallery

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  WB analysis using 24512-1-AP

  Mouse brain tissue was subjected to SDS PAGE, followed by western blot with 24512-1-AP (STXBP5 antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 24512-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24512-1-AP (STXBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 24512-1-AP

  IP result of anti-STXBP5 (IP:24512-1-AP, 5ug; Detection:24512-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IF Staining of SH-SY5Y using 24512-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24512-1-AP (STXBP5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24512-1-AP targets STXBP5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STXBP5 fusion protein Ag21629
• Full Name: syntaxin binding protein 5 (tomosyn)
• Calculated Molecular Weight: 1151 aa, 128 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC113382
• Gene Symbol: STXBP5
• Gene ID (NCBI): 134957
• RRID: AB_2879582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-binding protein 5 (STXBP5, also known as tomosyn) is a cytosolic syntaxin-binding protein that can displace MUNC18 from syntaxin-1. It is a soluble R-SNARE protein that sequesters target SNAREs through the C-terminal VAMP-like domain. STXBP5 plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release.

Publications

Species	Application	Title
human	WB	Sci Rep; The syntaxin-binding protein STXBP5 regulates progerin expression; Authors - Hongqian Qi ; KD Validated