SUPT6H Polyclonal antibody

SUPT6H Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (3)

Cat no : 23073-1-AP

Synonyms

SPT6H, SPT6, KIAA0162, hSPT6, Histone chaperone suppressor of Ty6

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Validation Data Gallery

• 

  WB analysis of HeLa using 23073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23073-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23073-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23073-1-AP

  IP result of anti-SUPT6H (IP:23073-1-AP, 4ug; Detection:23073-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human stomach cancer using 23073-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

23073-1-AP targets SUPT6H in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUPT6H fusion protein Ag19426
• Full Name: suppressor of Ty 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1726 aa, 199 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC136524
• Gene Symbol: SUPT6H
• Gene ID (NCBI): 6830
• RRID: AB_11232593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

The antibody detects SUPT6H from HeLa and Jurkat cells by WB (1/1000 dilution - Overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa/Jurkat

FH

Fabienne (Verified Customer) (06-07-2024)

Works very well in IP and Western blot

• Applications: Western Blot, Immunoprecipitation

FH

Mrinmoyee (Verified Customer) (09-21-2018)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: WB: 1:1000, IHC: 1:100, IF: 1:100, IP: 4ug
• Cell Tissue Type: Cancer cells and tissues

SUPT6H Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation, validation (WB: 1:1000, IHC: 1:100, IF: 1:100, IP: 4ug dilution) in Cancer cells and tissues (Cat no:23073-1-AP)