SYK Monoclonal antibody

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• SYK Monoclonal antibody (66721-1-Ig)

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SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 66721-1-Ig Copied!

Clone No. 4C4A12

Publications (9)

Download datasheet

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, IP, ELISA
For FC, select conjugated version.

Synonyms

4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase

• 

  WB analysis using 66721-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66721-1-Ig (SYK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of THP-1 using 66721-1-Ig

  THP-1 (WT and SYK KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 66721-1-Ig incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of Raji using 66721-1-Ig

  WB result of SYK antibody (66721-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MDA-MB-231 using 66721-1-Ig

  MDA-MB-231 lysates prepared and IP of SYK performed using 2.0 μg of 66721-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IHC staining of human tonsillitis using 66721-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66721-1-Ig (SYK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 66721-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66721-1-Ig (SYK antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells, THP-1 cells, Ramos cells, Daudi cells
• Positive IP detected in: MDA-MB-231 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66721-1-Ig targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17654

  Product name: Recombinant human SYK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 239-427 aa of BC001645

  Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL

• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 635 aa, 72 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• ENSEMBL Gene ID: ENSG00000165025
• RRID: AB_2882072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43405
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Publications

What published studies show

The SYK Monoclonal antibody (catalog number 66721-1-Ig) has 9 total citations. These appear in journals including Cancer Cell, Cancer Letters, Cell Reports, Cell Biology and Toxicology, PLoS Pathogens, Experimental Cell Research, Gene, and F1000Research. The associated research fields span oncology (glioblastoma, pancreatic cancer, hepatocellular carcinoma, acute myeloid leukemia), immunology and inflammation (lupus nephritis, inflammasome activation, diabetic retinopathy), and antibody validation for Western blot, immunoprecipitation, and immunofluorescence applications.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cancer Cell; Infiltrating plasma cells maintain glioblastoma stem cells through IgG-Tumor binding; Authors - Jiancheng Gao
mouse	WB	Cancer Cell; Distinct roles of TREM2 in central nervous system cancers and peripheral cancers; Authors - Jian Zhong
mouse	WB	Cancer Lett; SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.; Authors - Tian-Jiao Li
mouse	WB,IF	Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian
		Cell Biol Toxicol; Troxerutin suppresses the stemness of hepatocellular carcinoma via the Syk/FOXO3 feedback loop.; Authors - Shan Liu
pig,mouse	WB	PLoS Pathog; Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation; Authors - Xiaohua Zhang