SYK Monoclonal antibody, PBS Only

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  WB analysis using 66721-1-Ig (same clone as 66721-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66721-1-Ig (SYK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66721-1-Ig (same clone as 66721-1-PBS)

  WB result of SYK antibody (66721-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66721-1-Ig (same clone as 66721-1-PBS)

  THP-1 (WT and SYK KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 66721-1-Ig incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IP experiment of MDA-MB-231 using 66721-1-Ig (same clone as 66721-1-PBS)

  MDA-MB-231 lysates prepared and IP of SYK performed using 2.0 μg of 66721-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66721-1-Ig (SYK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66721-1-Ig (SYK antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 66721-1-PBS

Clone No.4C4A12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase

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Product Information

66721-1-PBS targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17654

  Product name: Recombinant human SYK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 239-427 aa of BC001645

  Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL

• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 635 aa, 72 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• ENSEMBL Gene ID: ENSG00000165025
• RRID: AB_2882072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43405
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)