SYNCRIP Polyclonal antibody

SYNCRIP Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30443-1-AP

Synonyms

dJ3J17.2, GRY RBP, hnRNP Q, HNRPQ, HNRPQ1, NS1 associated protein 1, NSAP1, pp68, RP1 3J17.2, SYNCRIP

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Validation Data Gallery

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  WB analysis using 30443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30443-1-AP (SYNCRIP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30443-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (30443-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, THP-1 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30443-1-AP targets SYNCRIP in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNCRIP fusion protein Ag31509
• Full Name: synaptotagmin binding, cytoplasmic RNA interacting protein
• Calculated Molecular Weight: 623 aa, 70 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC032643
• Gene Symbol: SYNCRIP
• Gene ID (NCBI): 10492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).