SYT2 Polyclonal antibody

SYT2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21245-1-AP

Synonyms

Synaptotagmin 2, synaptotagmin II, SYT2, SytII

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Validation Data Gallery

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  WB analysis using 21245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cerebellum using 21245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 21245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a cells
• Positive IF detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21245-1-AP targets SYT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYT2 fusion protein Ag15744
• Full Name: synaptotagmin II
• Calculated Molecular Weight: 419 aa, 47 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC100815
• Gene Symbol: SYT2
• Gene ID (NCBI): 127833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).