SYTL5 Polyclonal antibody

• 

  IHC staining of mouse brain using 24528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24528-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24528-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 24528-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SYTL5 antibody (24528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SYTL5 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 24528-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

slp5, synaptotagmin like 5, Synaptotagmin like protein 5, SYTL5

---

Tested Applications

• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24528-1-AP targets SYTL5 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYTL5 fusion protein Ag21698
• Full Name: synaptotagmin-like 5
• Calculated Molecular Weight: 730 aa, 81 kDa
• GenBank Accession Number: BC131585
• Gene Symbol: SYTL5
• Gene ID (NCBI): 94122
• RRID: AB_3669441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TDW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).