Septin 6 Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 12805-1-AP (same clone as 12805-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12805-1-AP (same clone as 12805-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

  IP result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Septin 6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12805-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

12805-1-PBS targets Septin 6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3521

  Product name: Recombinant human SEPT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 86-434 aa of BC036240

  Sequence: YDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE

• Full Name: septin 6
• Calculated Molecular Weight: 434 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036240
• Gene Symbol: SEPT6
• Gene ID (NCBI): 23157
• RRID: AB_2184993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14141
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.