Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12805-1-AP targets Septin 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3521 Product name: Recombinant human SEPT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 86-434 aa of BC036240 Sequence: YDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE Predict reactive species |
| Full Name | septin 6 |
| Calculated Molecular Weight | 434 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC036240 |
| Gene Symbol | SEPT6 |
| Gene ID (NCBI) | 23157 |
| RRID | AB_2184993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14141 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| IP protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| WB protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SEPT6 antibody (Cat# 12805-1-AP) has 17 citations spanning journals including Brain, Cell Death & Disease, Cell Reports, Cell Chemical Biology, Molecular Biology of the Cell, Journal of Biological Chemistry, Lab Investigation, FEBS Letters, Molecular Human Reproduction, Molecular Cell Proteomics, Proteomics, Cytoskeleton, Scientific Reports, International Journal of Oncology, Science China Life Sciences, Cells, and bioRxiv. Research fields cover septin cytoskeleton biology, cytokinesis, hepatocellular and prostate cancer, sperm biology, liver fibrosis, and plasma membrane repair.
| Species | Application | Title |
|---|---|---|
Brain Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease. | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
Sci China Life Sci Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes | ||
Cell Chem Biol The septin modifier, forchlorfenuron, activates NLRP3 via a potassium-independent mitochondrial axis | ||
Cell Rep An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis | ||
Int J Oncol SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway. |
Reviews
What customers say
The single review for this product reports a 2-star rating from an immunofluorescence experiment on testis tissue at a 1:200 dilution. The user noted that the antibody did not produce strong staining at the sperm annulus and exhibited very high background overall. No additional reviews are available for this catalog number.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mohammed (Verified Customer) (08-19-2020) | Did not show strong staining at the sperm annulus. Overall very high background.
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