Septin 6 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 12805-1-AP

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6

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Validation Data Gallery

• 

  WB analysis of Jurkat using 12805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12805-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12805-1-AP

  IP result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

12805-1-AP targets Septin 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 6 fusion protein Ag3521
• Full Name: septin 6
• Calculated Molecular Weight: 434 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036240
• Gene Symbol: SEPT6
• Gene ID (NCBI): 23157
• RRID: AB_2184993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

Publications

Species	Application	Title
human	WB	J Biol Chem; Uncovering principles that control septin-septin interactions.; Authors - Moshe S Kim
human,rat	WB	Lab Invest; Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.; Authors - Yuhui Fan ; KD Validated
mouse	WB	Proteomics; Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins.; Authors - She Yi-Min YM
human	IHC	Int J Oncol; SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway.; Authors - Yuhui Fan
human	WB	Mol Biol Cell; Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.; Authors - Forooz Soroor
human	IF	Mol Hum Reprod; The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.; Authors - Yi-Ru Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Did not show strong staining at the sperm annulus. Overall very high background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis