Serum amyloid P component Monoclonal antibody, PBS Only

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  WB analysis of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66084-1-Ig (same clone as 66084-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66084-1-PBS

Clone No.1A1D10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

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Product Information

66084-1-PBS targets Serum amyloid P component in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14957

  Product name: Recombinant human APCS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 20-223 aa of BC007058

  Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

• Full Name: amyloid P component, serum
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007058
• Gene Symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• RRID: AB_11182271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02743
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.