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  • Western Blot (WB) analysis of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human kidney using 66084-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human plasma using 66084-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of mouse spleen using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Serum amyloid P component Monoclonal antibody

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66084-1-Ig
Clone No.1A1D10
Publications(2)

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inhuman kidney tissue, human plasma
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human liver tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

66084-1-Ig targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14957

Product name: Recombinant human APCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-223 aa of BC007058

Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

Predict reactive species
Full Name amyloid P component, serum
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_11182271
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02743
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Protocols

Product Specific Protocols
IF protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
IHC protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
WB protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell

Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures

Authors - Yingjie Ding
View Article
humanIHC

Clin Chim Acta

Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC

Authors - Weifeng Wang
View Article
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