Streptavidin-HRP Conjugate

Applications

ELISA, WB, IHC

Conjugate

HRP

Host

Reactivity

Cat no : HRP-PF00030

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Validation Data Gallery

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  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 40X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). 一抗用的Biotin-conjugated Ki-67 Recombinant monoclonal antibody（批次：21024638）

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  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using HRP-PF00030 (HRP Streptavidin) at dilution of 1:500 (under 20X lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). 一抗用的Biotin-conjugated Ki-67 Recombinant monoclonal antibody（批次：21024638）

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  The antibodies (Biotin-98145-4、Biotin-FcA98138、Biotin-98051) were diluted 5-fold from an initial concentration of 2 ug/mL over 8 points on the plate that had been coated with the antigen, The plate was incubated at 37℃ for 1 hour, wash the plate three times. For the detection, HRP-PE00030: lot#21026977 was used at 1/2000 dilution (100 uL/well), followed by incubation for 1h at 37°C.

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Product Information

辣根过氧化物酶-链霉亲和素（HRP-Streptavidin HRP-PF00030）偶联物，是链霉亲和素（SA）与辣根过氧化物酶（HRP）偶联的产物。这种高度敏感的检测器结合可以用于生物素（Biotin）标记的抗体、核酸、蛋白或其它生物素标记分子的检测，具体用途包括Western、ELISA、IHC等。本产品 Streptavidin-HRP（HRP-PF00030）用于各种常规用途的推荐稀释比例参考右表，实际实验操作过程中需根据具体情况适当调节稀释比例。

• Concentration: 0.2 mg/mL
• Applications: ELISA, WB, IHC
• Physical State: Liquid
• Suggested Dilution Range: 1:1000 for western blotting;; 1:100 for immunohistochemistry;; 1:1000 for ELISA.
• Storage Buffer: PBS with 0.1% Proclin 300, pH 7.4.
• Storage Instructions: 2-8℃ for one year.