TARS2 Polyclonal antibody

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  WB analysis of HepG2 using 15067-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15067-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15067-1-AP (TARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15067-1-AP

  IP result of anti-TARS2 (IP:15067-1-AP, 4ug; Detection:15067-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse heart using 15067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15067-1-AP (TARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15067-1-AP (TARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15067-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Threonyl-tRNA synthetase-like 1, Threonyl-tRNA synthetase, Threonine--tRNA ligase, mitochondrial, Threonine  tRNA ligase, TARSL1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse heart tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15067-1-AP targets TARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TARS2 fusion protein Ag7074
• Full Name: threonyl-tRNA synthetase 2, mitochondrial (putative)
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC000541
• Gene Symbol: TARS2
• Gene ID (NCBI): 80222
• RRID: AB_2200668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BW92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF, WB, IP	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim ; KD Validated
human	WB	Bioengineered; Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis.; Authors - Hui Tian ; KD Validated
mouse	WB	Redox Biol; Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle; Authors - Iliana López-Soldado
human	IHC	Int J Biol Markers; Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation; Authors - Lida Suo