• Featured Product
  • KD/KO Validated

TAZ Monoclonal antibody

TAZ Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A12A10

Cat no : 66500-1-Ig

Synonyms

TAZ, WWTR1



Tested Applications

Positive WB detected inMCF-7 cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, MDA-MB-453S cells, Hela cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66500-1-Ig targets TAZ in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAZ fusion protein Ag13330
Full Name WW domain containing transcription regulator 1
Calculated molecular weight 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC014052
Gene symbol WWTR1
Gene ID (NCBI) 25937
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protocols

Product Specific Protocols
WB protocol for TAZ antibody 66500-1-IgDownload protocol
IHC protocol for TAZ antibody 66500-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Transl Res

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

Authors - Adheesh Bhandari
humanWB

J Exp Clin Cancer Res

Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.

Authors - Ming-Xin Cao