Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, 4T1 cells, MDA-MB-453S cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66500-1-Ig targets WWTR1/TAZ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13330 Product name: Recombinant human WWTR1,TAZ protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-400 aa of BC014052 Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL Predict reactive species |
| Full Name | WW domain containing transcription regulator 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC014052 |
| Gene Symbol | WWTR1 |
| Gene ID (NCBI) | 25937 |
| RRID | AB_2881864 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9GZV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WWTR1/TAZ antibody 66500-1-Ig | Download protocol |
| IHC protocol for WWTR1/TAZ antibody 66500-1-Ig | Download protocol |
| WB protocol for WWTR1/TAZ antibody 66500-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The YAP antibody (Cat# 66500-1-Ig) has 24 citations across journals including Nature Communications, EMBO Journal, Developmental Cell, Cell Death & Differentiation, Autophagy, Leukemia, and Cell Signaling. Research fields span the Hippo/YAP signaling pathway in oncology (glioblastoma, renal cell carcinoma, esophageal carcinoma, hepatocellular carcinoma, prostate cancer, thyroid cancer, leukemia), neurobiology (Alzheimer's disease, neurogenesis, spinal cord injury), bone biology (osteoporosis, osteogenesis), fibrosis (pulmonary, vascular, intestinal), and cartilage degeneration. Applications include WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Autophagy Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis. | ||
Nat Commun LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.
| ||
J Exp Clin Cancer Res Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression. | ||
Cell Death Differ Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma | ||
Cancer Biol Med The polarity protein Par3 enhances renal cell carcinoma metastasis via YAP/TAZ activation | ||
Dev Cell STAT3-controlled CHI3L1/SPP1 positive feedback loop demonstrates the spatial heterogeneity and immune characteristics of glioblastoma |
Reviews
What customers say
The single review for 66500-1-Ig is a 5-star rating from a researcher who used it at a 1/500 dilution for immunofluorescence on vascular smooth muscle cells. They reported that the immunostaining worked well. Overall, the feedback is positive, indicating reliable performance for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rumeyza (Verified Customer) (03-26-2026) | the immunostaining worked well.
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