WWTR1/TAZ Monoclonal antibody

• Featured Product
• KD/KO Validated

WWTR1/TAZ Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

2A12A10

Cat no : 66500-1-Ig

Synonyms

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, 2A12A10

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Validation Data Gallery

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  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66500-1-Ig

  WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells.

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  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66500-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66500-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66500-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, 4T1 cells, MDA-MB-453S cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

66500-1-Ig targets WWTR1/TAZ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WWTR1/TAZ fusion protein Ag13330
• Full Name: WW domain containing transcription regulator 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014052
• Gene Symbol: WWTR1
• Gene ID (NCBI): 25937
• RRID: AB_2881864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma; Authors - Yu-Fei Cao
human	WB	Nat Commun; LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.; Authors - Huai-Bin Hu ; KD Validated
mouse	WB,IF	Cell Signal; RhoA/ROCK-TAZ Axis regulates bone formation within calvarial trans-sutural distraction osteogenesis; Authors - Junjie Ye
mouse	WB,IHC,IF	Front Pharmacol; TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.; Authors - Xuyang Hu
human	WB	Am J Transl Res; VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.; Authors - Adheesh Bhandari
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen