|Positive WB detected in||MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, MDA-MB-453S cells, Hela cells, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
66500-1-Ig targets TAZ in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Tested Reactivity||Human, rat, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||TAZ fusion protein Ag13330|
|Full Name||WW domain containing transcription regulator 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC014052|
|Gene ID (NCBI)||25937|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.
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