WWTR1/TAZ Monoclonal antibody, PBS Only

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  WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

  WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66500-1-Ig (same clone as 66500-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66500-1-Ig (same clone as 66500-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66500-1-Ig (same clone as 66500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66500-1-Ig (same clone as 66500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

WWTR1/TAZ Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66500-1-PBS

Clone No.2A12A10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TAZ, WWTR1, 2A12A10, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1

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Product Information

66500-1-PBS targets WWTR1/TAZ in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13330

  Product name: Recombinant human WWTR1,TAZ protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-400 aa of BC014052

  Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

• Full Name: WW domain containing transcription regulator 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014052
• Gene Symbol: WWTR1
• Gene ID (NCBI): 25937
• RRID: AB_2881864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9GZV5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.