TBC1D1 Polyclonal antibody, PBS Only

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  WB analysis of LNCaP using 22124-1-AP (same clone as 22124-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 22124-1-AP (same clone as 22124-1-PBS)

  WB result of TBC1D1 antibody (22124-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D1 transfected HEK-293T cells. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22124-1-AP (same clone as 22124-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 22124-1-AP (same clone as 22124-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 22124-1-AP (same clone as 22124-1-PBS)

  IP result of anti-TBC1D1 (IP:22124-1-AP, 4ug; Detection:22124-1-AP 1:1000) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 22124-1-AP (same clone as 22124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22124-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TBC1D1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 22124-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

22124-1-PBS targets TBC1D1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5406

  Product name: Recombinant human TBC1D1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 813-1168 aa of BC050321

  Sequence: LAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD

• Full Name: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 150-165 kDa
• GenBank Accession Number: BC050321
• Gene Symbol: TBC1D1
• Gene ID (NCBI): 23216
• RRID: AB_2827874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TI0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.