Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse kidney tissue, human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
22124-1-AP targets TBC1D1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5406 Product name: Recombinant human TBC1D1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 813-1168 aa of BC050321 Sequence: LAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD Predict reactive species |
| Full Name | TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 |
| Calculated Molecular Weight | 133 kDa |
| Observed Molecular Weight | 150-165 kDa |
| GenBank Accession Number | BC050321 |
| Gene Symbol | TBC1D1 |
| Gene ID (NCBI) | 23216 |
| RRID | AB_2827874 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| IP protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| WB protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise | ||
Diabetes Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle. | ||
Mol Nutr Food Res Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation. | ||
J Physiol The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. | ||
J Biol Chem Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Q (Verified Customer) (06-01-2021) | Excellent to detect TBC1D1 in postmortem human samples with WB with the right MW.
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