TBC1D4 Monoclonal antibody

• 

  WB analysis using 68063-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68063-1-Ig (TBC1D4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TBC1D4 Monoclonal Antibody for WB, ELISA

Cat No. 68063-1-Ig

Clone No.1E2H2

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, CoIP, ELISA

Synonyms

Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HCT 116 cells, LNCaP cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

68063-1-Ig targets TBC1D4 in WB, IF, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20341

  Product name: Recombinant human TBC1D4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-200 aa of BC151239

  Sequence: MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQ

• Full Name: TBC1 domain family, member 4
• Calculated Molecular Weight: 1299 aa, 147 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC151239
• Gene Symbol: TBC1D4
• Gene ID (NCBI): 9882
• RRID: AB_2918804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60343
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IF,CoIP	Nat Commun; Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation; Authors - Carlos Antón-Plágaro