TBCA Polyclonal antibody

TBCA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12304-1-AP

Synonyms

CFA, TBCA, TCP1 chaperonin cofactor A, tubulin folding cofactor A, Tubulin specific chaperone A



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12304-1-AP targets TBCA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBCA fusion protein Ag2950
Full Name tubulin folding cofactor A
Calculated Molecular Weight 108 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC018210
Gene Symbol TBCA
Gene ID (NCBI) 6902
RRIDAB_2199402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBCA antibody 12304-1-APDownload protocol
IHC protocol for TBCA antibody 12304-1-APDownload protocol
IP protocol for TBCA antibody 12304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Cancer

Tubulin cofactor a functions as a novel positive regulator of ccRCC progression, invasion and metastasis.

Authors - Zhang Peng P
mouseIF

Histochem Cell Biol

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.

Authors - Lukas Juergens
humanWB

Front Genet

Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.

Authors - Hongxia Yuan
humanWB

Oncol Rep

ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer.

Authors - Lili Guo