TBCA Polyclonal antibody

TBCA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12304-1-AP

Synonyms

CFA, TBCA, TCP1 chaperonin cofactor A, tubulin folding cofactor A, Tubulin specific chaperone A

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Validation Data Gallery

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  WB analysis of human brain using 12304-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12304-1-AP (TBCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12304-1-AP (TBCA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12304-1-AP

  IP result of anti-TBCA (IP:12304-1-AP, 4ug; Detection:12304-1-AP 1:300) with HeLa cells lysate 2920 ug.

• 

  IHC staining of human prostate cancer using 12304-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12304-1-AP (TBCA antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12304-1-AP targets TBCA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBCA fusion protein Ag2950
• Full Name: tubulin folding cofactor A
• Calculated Molecular Weight: 108 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC018210
• Gene Symbol: TBCA
• Gene ID (NCBI): 6902
• RRID: AB_2199402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Int J Cancer; Tubulin cofactor a functions as a novel positive regulator of ccRCC progression, invasion and metastasis.; Authors - Zhang Peng P
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens
human	WB	Front Genet; Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.; Authors - Hongxia Yuan
human	WB	Oncol Rep; ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer.; Authors - Lili Guo