TCEAL7 Polyclonal antibody

• Featured Product
• KD/KO Validated

TCEAL7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11218-1-AP

Synonyms

MPMGp800C04260Q003, TCEA like protein 7, TCEAL7

---

Validation Data Gallery

• 

  WB analysis of human liver using 11218-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11218-1-AP (TCEAL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11218-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11218-1-AP (TCEAL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 11218-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11218-1-AP (TCEAL7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11218-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11218-1-AP (TCEAL7 antibody) at dilution of 1:50 (under 25x lens).

---

Tested Applications

• Positive WB detected in: human liver tissue, A549 cells
• Positive IHC detected in: human ovary tumor tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11218-1-AP targets TCEAL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCEAL7 fusion protein Ag1715
• Full Name: transcription elongation factor A (SII)-like 7
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC016786
• Gene Symbol: TCEAL7
• Gene ID (NCBI): 56849
• RRID: AB_2201131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEAL7 is a transcription regulatory factor that has a role in the negative regulation of NF-kappa-B signaling at the basal level by regulating transcriptional activity of NF-kappa-B on its target gene promoters. It can associate with cyclin D1 promoter containing Myc E-box sequence and transcriptionally represses cyclin D1 expression. In addition, TCEAL7 regulates telomerase reverse transcriptase expression and telomerase activity in both ALT (alternative lengthening of telomeres)and telomerase-positive cell lines

Publications

Species	Application	Title
human	WB	Cancer Cell Int; Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1.; Authors - Xilin Liu ; KD Validated
human	WB,IHC	PLoS One; Decreased expression of transcription elongation factor A-like 7 is associated with gastric adenocarcinoma prognosis.; Authors - Huang Chun-yu CY