TCEB1 Polyclonal antibody

TCEB1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12450-1-AP

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12450-1-AP targets TCEB1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB1 fusion protein Ag3087
Full Name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Calculated molecular weight 112 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC013809
Gene symbol TCEB1
Gene ID (NCBI) 6921
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCEB1, also named as Elongin 15 kDa subunit or RNA polymerase II transcription factor SIII subunit C, is a 112 amino acid protein, which belongs to the SKP1 family. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Protocols

Product Specific Protocols
WB protocol for TCEB1 antibody 12450-1-APDownload protocol
IHC protocol for TCEB1 antibody 12450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Evid Based Complement Alternat Med

Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.

Authors - Chong-Chong Guo
humanIHC

Am J Pathol

A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression.

Authors - Agell Laia L