TCEB1 Polyclonal antibody

TCEB1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12450-1-AP

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12450-1-AP targets TCEB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB1 fusion protein Ag3087
Full Name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Calculated Molecular Weight 112 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC013809
Gene Symbol TCEB1
Gene ID (NCBI) 6921
RRIDAB_2201139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

Protocols

Product Specific Protocols
WB protocol for TCEB1 antibody 12450-1-APDownload protocol
IHC protocol for TCEB1 antibody 12450-1-APDownload protocol
IF protocol for TCEB1 antibody 12450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang
humanIHC

Am J Pathol

A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression.

Authors - Agell Laia L
ratWB

Evid Based Complement Alternat Med

Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.

Authors - Chong-Chong Guo
humanWB

bioRxiv

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska