TCEB2 Polyclonal antibody

TCEB2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10779-1-AP

Synonyms

ELOB, Elongin 18 kDa subunit, Elongin B, SIII, SIII p18, TCEB2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, K-562 cells, mouse skeletal muscle tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10779-1-AP targets TCEB2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB2 fusion protein Ag1228
Full Name transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
Calculated molecular weight 13 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC013306
Gene symbol TCEB2
Gene ID (NCBI) 6923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCEB2 antibody 10779-1-APDownload protocol
IHC protocol for TCEB2 antibody 10779-1-APDownload protocol
IP protocol for TCEB2 antibody 10779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.

Authors - Jiaxiang Chen