TCEB2/Elongin-B Polyclonal antibody

TCEB2/Elongin-B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10779-1-AP

Synonyms

ELOB, Elongin 18 kDa subunit, Elongin B, SIII, SIII p18, TCEB2



Tested Applications

Positive WB detected inHL-60 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

10779-1-AP targets TCEB2/Elongin-B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB2/Elongin-B fusion protein Ag1228
Full Name transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
Calculated molecular weight 13 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC013306
Gene symbol TCEB2
Gene ID (NCBI) 6923
RRIDAB_2240375
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEB2 encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex and is also reported to function as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes (PMID: 26531153). TCEB2 has 2 isoforms with the molecular mass of 13 and 18 kDa.

Protocols

Product Specific Protocols
WB protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
IHC protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
IP protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang
humanWB

Cell Signal

pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.

Authors - Jiaxiang Chen
humanWB

bioRxiv

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
humanWB

bioRxiv

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

Authors - Avi J Samelson