TCN2 Polyclonal antibody, PBS Only

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  WB analysis using 12157-1-AP (same clone as 12157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 12157-1-AP (same clone as 12157-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

TCN2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12157-1-PBS targets TCN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2825

  Product name: Recombinant human TCN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC011239

  Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW

• Full Name: transcobalamin II; macrocytic anemia
• Calculated Molecular Weight: 400 aa, 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC011239
• Gene Symbol: TCN2
• Gene ID (NCBI): 6948
• RRID: AB_2303252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20062
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)