|Positive WB detected in||BxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells|
|Positive IHC detected in||mouse small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:300-1:600|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12157-1-AP targets TCN2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, cat, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TCN2 fusion protein Ag2825|
|Full Name||transcobalamin II; macrocytic anemia|
|Calculated molecular weight||400 aa, 44 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC011239|
|Gene ID (NCBI)||6948|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)
Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.
Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joseph (Verified Customer) (09-17-2020)
We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies