TCN2 Polyclonal antibody

TCN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 12157-1-AP


D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

Tested Applications

Positive WB detected inBxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12157-1-AP targets TCN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, cat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825
Full Name transcobalamin II; macrocytic anemia
Calculated Molecular Weight 400 aa, 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011239
Gene Symbol TCN2
Gene ID (NCBI) 6948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)


Product Specific Protocols
WB protocol for TCN2 antibody 12157-1-APDownload protocol
IHC protocol for TCN2 antibody 12157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


canine, catIHC


Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Authors - Annette M Sysel

Int J Biol Macromol

CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.

Authors - Yue Chen

Anticancer Res

Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.

Authors - Sysel Annette M AM

Mol Pharm

Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin

Authors - Yuri Kinoshita


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Joseph (Verified Customer) (09-17-2020)

We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
  • Cell Tissue Type: human, canine and feline malignant various