Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells |
| Positive IHC detected in | mouse small intestine tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12157-1-AP targets TCN2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, cat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2825 Product name: Recombinant human TCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC011239 Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW Predict reactive species |
| Full Name | transcobalamin II; macrocytic anemia |
| Calculated Molecular Weight | 400 aa, 44 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC011239 |
| Gene Symbol | TCN2 |
| Gene ID (NCBI) | 6948 |
| RRID | AB_2303252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TCN2 antibody 12157-1-AP | Download protocol |
| WB protocol for TCN2 antibody 12157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 12157-1-AP targets CD320 (TCN2), the vitamin B₁₂ cell-surface receptor. It has 5 total citations published in Int J Biol Macromol, Oncotarget, Mol Pharm, FASEB J, and Anticancer Res. Research fields span renal and intestinal epithelial biology, oncology (canine/feline tumors and human xenografts), vitamin B₁₂ transport across the blood-retinal barrier, and psoriasis-related inflammation involving IL-1β/STAT3 signaling. Applications include IF, IHC, and WB across mouse, canine, feline, rat, and human samples.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol CD320 expression and apical membrane targeting in renal and intestinal epithelial cells. | ||
Oncotarget Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues. | ||
Mol Pharm Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin | ||
FASEB J TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation. | ||
Anticancer Res Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts. |
Reviews
What customers say
The single review for this antibody (a 5-star rating) reports that the user evaluated several TCN2 antibodies from different manufacturers and found this Proteintech sequence to offer the best sensitivity and specificity. It was used for immunohistochemistry on human, canine, and feline malignant tissues at 1:50 dilution (human and canine) and 1:25 (feline). Overall, the feedback is positive, highlighting superior performance compared to competing products.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joseph (Verified Customer) (09-17-2020) | We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies
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