TCN2 Polyclonal antibody

TCN2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12157-1-AP

Synonyms

D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II



Tested Applications

Positive WB detected inBxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

12157-1-AP targets TCN2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, cat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCN2 fusion protein Ag2825
Full Name transcobalamin II; macrocytic anemia
Calculated molecular weight 400 aa, 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011239
Gene symbol TCN2
Gene ID (NCBI) 6948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

Protocols

Product Specific Protocols
WB protocol for TCN2 antibody 12157-1-APDownload protocol
IHC protocol for TCN2 antibody 12157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Anticancer Res

Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.

Authors - Sysel Annette M AM
canine, catIHC

Oncotarget

Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Authors - Annette M Sysel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
  • Cell Tissue Type: human, canine and feline malignant various