TFAM Recombinant antibody

TFAM Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 82745-1-RR

Synonyms

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Validation Data Gallery

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  WB analysis using 82745-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82745-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82745-1-RR targets TFAM in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 24-25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.