TFB1M Polyclonal antibody

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• TFB1M Polyclonal antibody (16604-1-AP)

• 

  WB analysis of HepG2 using 16604-1-AP

  WB result of TFB1M antibody (16604-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFB1M transfected HepG2 cells.

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  IP experiment of HepG2 using 16604-1-AP

  IP result of anti-TFB1M (IP:16604-1-AP, 4ug; Detection:16604-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

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  IF Staining of HepG2 using 16604-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFB1M antibody (16604-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TFB1M Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 16604-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

CGI 75, CGI75, CGI-75, EC:2.1.1.-, h mtTFB

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Tested Applications

• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16604-1-AP targets TFB1M in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9785

  Product name: Recombinant human TFB1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC017788

  Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

• Full Name: transcription factor B1, mitochondrial
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC017788
• Gene Symbol: TFB1M
• Gene ID (NCBI): 51106
• RRID: AB_3085501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVM0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)