TFB1M Polyclonal antibody

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  WB analysis of HepG2 using 16604-1-AP

  WB result of TFB1M antibody (16604-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFB1M transfected HepG2 cells.

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  IP experiment of HepG2 using 16604-1-AP

  IP result of anti-TFB1M (IP:16604-1-AP, 4ug; Detection:16604-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

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  IF Staining of HepG2 using 16604-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFB1M antibody (16604-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TFB1M Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 16604-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

CGI 75, CGI75, CGI-75, EC:2.1.1.-, h mtTFB

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Tested Applications

• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16604-1-AP targets TFB1M in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9785

  Product name: Recombinant human TFB1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC017788

  Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

• Full Name: transcription factor B1, mitochondrial
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC017788
• Gene Symbol: TFB1M
• Gene ID (NCBI): 51106
• RRID: AB_3085501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVM0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.