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  • KD/KO Validated

TFCP2 Polyclonal antibody

TFCP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15203-1-AP

Synonyms

CP2, LBP 1C, LSF, SAA3 enhancer factor, SEF, TFCP2, TFCP2C, transcription factor CP2, Transcription factor LSF



Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15203-1-AP targets TFCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFCP2 fusion protein Ag7353
Full Name transcription factor CP2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC003634
Gene Symbol TFCP2
Gene ID (NCBI) 7024
RRIDAB_2199582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)

Protocols

Product Specific Protocols
WB protocol for TFCP2 antibody 15203-1-APDownload protocol
IP protocol for TFCP2 antibody 15203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Pollut

Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice.

Authors - Ruyue Fang
mouseWB

Cell Death Dis

MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells.

Authors - Jiawei Zhou
humanIP

Clin Epigenetics

DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter.

Authors - Keke Shao
humanWB,IHC

Front Oncol

TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer.

Authors - Dexiang Zhang
  • KD Validated
humanWB

Exp Cell Res

TFCP2, a binding protein of ATF3, promotes the progression of glioma by activating the synthesis of serine.

Authors - Xiangying Luo
  • KD Validated
human,mouseWB,IF

J Exp Clin Cancer Res

Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis.

Authors - Hongkun Cai