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  • KD/KO Validated

TFCP2 Polyclonal antibody

TFCP2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15203-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

CP2, LBP 1C, LSF, SAA3 enhancer factor, SEF, TFCP2, TFCP2C, transcription factor CP2, Transcription factor LSF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15203-1-AP targets TFCP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7353

Product name: Recombinant human TFCP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC003634

Sequence: MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRL

Predict reactive species
Full Name transcription factor CP2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC003634
Gene Symbol TFCP2
Gene ID (NCBI) 7024
RRIDAB_2199582
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12800
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)

Protocols

Product Specific Protocols
WB protocol for TFCP2 antibody 15203-1-APDownload protocol
IP protocol for TFCP2 antibody 15203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Pollut

Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice.

Authors - Ruyue Fang
mouseWB

Cell Death Dis

MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells.

Authors - Jiawei Zhou
humanIP

Clin Epigenetics

DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter.

Authors - Keke Shao
humanWB,IHC

Front Oncol

TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer.

Authors - Dexiang Zhang
  • KD Validated
humanWB

Exp Cell Res

TFCP2, a binding protein of ATF3, promotes the progression of glioma by activating the synthesis of serine.

Authors - Xiangying Luo
  • KD Validated
human,mouseWB,IF

J Exp Clin Cancer Res

Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis.

Authors - Hongkun Cai
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