Tested Applications
| Positive WB detected in | mouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells |
| Positive IP detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15203-1-AP targets TFCP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7353 Product name: Recombinant human TFCP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC003634 Sequence: MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRL Predict reactive species |
| Full Name | transcription factor CP2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC003634 |
| Gene Symbol | TFCP2 |
| Gene ID (NCBI) | 7024 |
| RRID | AB_2199582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12800 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| WB protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TFCP2 antibody (Cat# 15203-1-AP) has 9 total citations published in journals including Mol Cell, J Exp Clin Cancer Res, Cell Death Dis, Environ Pollut, Front Immunol, Clin Epigenetics, Vascul Pharmacol, Exp Cell Res, and Front Oncol. Research fields span STING-mediated lysosomal biogenesis, pancreatic cancer, ovarian cell biology, lipid homeostasis, colon cancer epigenetics, colorectal cancer metastasis, ferroptosis, glioma progression, and cholesterol synthesis.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis. | ||
Cell Death Dis MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells. | ||
Environ Pollut Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice. | ||
Front Immunol The genetic and epigenetic regulation of CD55 and its pathway analysis in colon cancer
| ||
Clin Epigenetics DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter. |
