TFCP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TFCP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15203-1-AP

Synonyms

CP2, LBP 1C, LSF, SAA3 enhancer factor, SEF, TFCP2, TFCP2C, transcription factor CP2, Transcription factor LSF

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Validation Data Gallery

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  WB analysis of mouse spleen using 15203-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15203-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15203-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15203-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15203-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15203-1-AP (TFCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 15203-1-AP

  IP result of anti-TFCP2 (IP:15203-1-AP, 4ug; Detection:15203-1-AP 1:500) with mouse spleen tissue lysate 4000 ug.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15203-1-AP targets TFCP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFCP2 fusion protein Ag7353
• Full Name: transcription factor CP2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC003634
• Gene Symbol: TFCP2
• Gene ID (NCBI): 7024
• RRID: AB_2199582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice.; Authors - Ruyue Fang
mouse	WB	Cell Death Dis; MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells.; Authors - Jiawei Zhou
human	IP	Clin Epigenetics; DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter.; Authors - Keke Shao
human	WB,IHC	Front Oncol; TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer.; Authors - Dexiang Zhang ; KD Validated
human	WB	Exp Cell Res; TFCP2, a binding protein of ATF3, promotes the progression of glioma by activating the synthesis of serine.; Authors - Xiangying Luo ; KD Validated
human,mouse	WB,IF	J Exp Clin Cancer Res; Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis.; Authors - Hongkun Cai