TFEB Polyclonal antibody

• 

  WB analysis using 13372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13372-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 13372-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13372-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 13372-1-AP

  IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13372-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13372-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFEB antibody (13372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

The TFEB polyclonal antibody (13372-1-AP) is one of the most highly cited TFEB antibodies in the market. It is suitable for WB, IHC, IF/ICC, IP, ELISA and has been referenced in over 236 publications.

Cat No. 13372-1-AP

Publications(236)

Reviews (7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB

---

Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human renal cell carcinoma tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 29 publications below
• WB: See 190 publications below
• IHC: See 21 publications below
• IF: See 110 publications below
• IP: See 7 publications below
• CoIP: See 3 publications below

Product Information

13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4194

  Product name: Recombinant human TFEB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-476 aa of BC032448

  Sequence: MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL

• Full Name: transcription factor EB
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC032448
• Gene Symbol: TFEB
• Gene ID (NCBI): 7942
• RRID: AB_2199611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	ACS Nano; A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.; Authors - Jingyi Liu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB,IP,IF	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB,IF	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB,IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Sheeny (Verified Customer) (09-02-2025)

Used in organoids and it works well. The specificity of TFEB is very good with the subcellular localization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

FH

Flaviane (Verified Customer) (07-30-2025)

Very strong and clean signal.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Polycystic Kidney (FFPE sections)

TFEB Antibody Immunohistochemistry validation (1:200 dilution) in Mouse Polycystic Kidney (FFPE sections) (Cat no:13372-1-AP)

FH

Stanislas (Verified Customer) (08-05-2024)

WB on HeLa lysates using 15µg of protein and nitrocellulose membranes, blockage in TBST-5%Milk 1h, Incubation in 2%BSA with TFEB antibody (1:3000) overnight at 4°C. Worked on the first trial. IF on HeLa fixed in 4%PFA for 15min, blocked 1h in 1XPBS, 5%NGS, 0.3% Triton X-100, incubates with TFEB antibody (1:100) in 1XPBS, 1%BSA, 0.3% Triton X-100 overnight at 4°C. Worked on first trial.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeLa cells

FH

Shunbin (Verified Customer) (07-17-2023)

bands look specific, but blurry and multiple.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: B cell lines

TFEB Antibody Western Blot validation (1:2000 dilution) in B cell lines (Cat no:13372-1-AP)

FH

Weihao (Verified Customer) (04-11-2022)

Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mouse BMDM

FH

Marina (Verified Customer) (11-22-2021)

2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
• Cell Tissue Type: primary mouse microglia

FH

Tom (Verified Customer) (02-19-2021)

HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

TFEB Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:13372-1-AP)