TFEB Polyclonal antibody

• Featured Product
• KD/KO Validated

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay

Conjugate

Unconjugated

Publications

197

Reviews (5)

Cat no : 13372-1-AP

Synonyms

Transcription factor EB, TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB

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Validation Data Gallery

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  WB analysis using 13372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13372-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13372-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 13372-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 13372-1-AP

  IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13372-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 27 publications below
• WB: See 165 publications below
• IHC: See 18 publications below
• IF: See 88 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Product Information

13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFEB fusion protein Ag4194
• Full Name: transcription factor EB
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC032448
• Gene Symbol: TFEB
• Gene ID (NCBI): 7942
• RRID: AB_2199611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publications

Species	Application	Title
mouse	WB	ACS Nano; A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.; Authors - Jingyi Liu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB,IF,IP	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB,IF	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB,IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Stanislas (Verified Customer) (08-05-2024)

WB on HeLa lysates using 15µg of protein and nitrocellulose membranes, blockage in TBST-5%Milk 1h, Incubation in 2%BSA with TFEB antibody (1:3000) overnight at 4°C. Worked on the first trial. IF on HeLa fixed in 4%PFA for 15min, blocked 1h in 1XPBS, 5%NGS, 0.3% Triton X-100, incubates with TFEB antibody (1:100) in 1XPBS, 1%BSA, 0.3% Triton X-100 overnight at 4°C. Worked on first trial.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeLa cells

FH

Shunbin (Verified Customer) (07-17-2023)

bands look specific, but blurry and multiple.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: B cell lines

TFEB Antibody Western Blot validation (1:2000 dilution) in B cell lines (Cat no:13372-1-AP)

FH

Weihao (Verified Customer) (04-11-2022)

Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mouse BMDM

FH

Marina (Verified Customer) (11-22-2021)

2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
• Cell Tissue Type: primary mouse microglia

FH

Tom (Verified Customer) (02-19-2021)

HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

TFEB Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:13372-1-AP)

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