CoraLite®594-conjugated TFEB Polyclonal antibody

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• KD/KO Validated

TFEB Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-13372

Synonyms

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-13372

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 TFEB antibody (CL594-13372) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-13372

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 TFEB antibody (CL594-13372) at dilution of 1:600.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-13372 targets TFEB in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFEB fusion protein Ag4194
• Full Name: transcription factor EB
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC032448
• Gene Symbol: TFEB
• Gene ID (NCBI): 7942
• RRID: AB_2919794
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publications

Species	Application	Title
	IF	Genet Med; De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood; Authors - Margot R F Reijnders