TFEB Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84675-5-RR (same clone as 84675-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84675-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84675-5-RR (same clone as 84675-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (84675-5-RR, Clone: 242093D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84675-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84675-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

TFEB Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84675-5-PBS

Clone No.242093D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242093D11, Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35

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Product Information

84675-5-PBS targets TFEB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4194

  Product name: Recombinant human TFEB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-476 aa of BC032448

  Sequence: MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL

• Full Name: transcription factor EB
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC032448
• Gene Symbol: TFEB
• Gene ID (NCBI): 7942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P19484
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).