TFG Monoclonal antibody

TFG Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66916-1-Ig

Synonyms

FLJ36137, Protein TFG, TF6, TFG, TRK fused gene, TRK fused gene protein, TRKT3

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Validation Data Gallery

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  WB analysis using 66916-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66916-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66916-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66916-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, A549 cells, MCF-7 cells, HEK-293 cells, PC-3 cells, LNCaP cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66916-1-Ig targets TFG in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFG fusion protein Ag27697
• Full Name: TRK-fused gene
• Calculated Molecular Weight: 400 aa, 43 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC023599
• Gene Symbol: TFG
• Gene ID (NCBI): 10342
• RRID: AB_2882243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.