TGFBR2 Monoclonal antibody

• Featured Product
• KD/KO Validated

TGFBR2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

CloneNo.

2D5H7

Reviews (1)

Cat no : 66636-1-Ig

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

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Validation Data Gallery

• 

  WB analysis using 66636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HEK-293T using 66636-1-Ig

  WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells.

• 

  WB analysis of U2OS using 66636-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66636-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66636-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66636-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of THP-1 using 66636-1-Ig

  1X10^6 THP-1 cells were intracellularly stained with 0.13 ug Anti-Human TGFBR2 (66636-1-Ig, Clone:2D5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, U2OS cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, NCCIT cells, L02 cells, K-562 cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

66636-1-Ig targets TGFBR2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGFBR2 fusion protein Ag25773
• Full Name: transforming growth factor, beta receptor II (70/80kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-80 kDa
• GenBank Accession Number: BC040499
• Gene Symbol: TGFBR2
• Gene ID (NCBI): 7048
• RRID: AB_2881995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Publications

Species	Application	Title
mouse	WB	Cell Rep Med; Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity; Authors - Yajie Sun
mouse,rat	IF,WB,IHC	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
rat	WB,IP	Br J Pharmacol; Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway.; Authors - Xiyue Zhang
rat	IHC	NPJ Regen Med; Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration; Authors - Yuting Niu
human,mouse	WB,IHC	Acta Pharmacol Sin; N-n-Butyl haloperidol iodide ameliorates liver fibrosis and hepatic stellate cell activation in mice.; Authors - Dai-Fei Shen ; KD Validated
human	WB,IP	Aging (Albany NY); A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer.; Authors - Xiaotian Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-15-2023)

This antibody worked well for labeling TGF beta receptor type 2 in mouse kidney. We were able to acquire robust immunofluorescence signals with this KO validated antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Kidney tissue

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C