THTPA Polyclonal antibody

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THTPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15486-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ThTPase, THTP, Thiamine-triphosphatase, thiamine triphosphatase, EC:3.6.1.28

• 

  WB analysis of K-562 using 15486-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15486-1-AP

  WB result of THTPA antibody (15486-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THTPA transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 15486-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 15486-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse uterus using 15486-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 15486-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 15486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 15486-1-AP

  IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15486-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15486-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15486-1-AP

  Immunofluorescent analysis of HepG2 cells, using THTPA antibody 15486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, A431 cells, HeLa cells, human brain tissue, mouse testis tissue, mouse uterus tissue, PC-3 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15486-1-AP targets THTPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7834

  Product name: Recombinant human THTPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-230 aa of BC002984

  Sequence: MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG

• Full Name: thiamine triphosphatase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26-35 kDa
• GenBank Accession Number: BC002984
• Gene Symbol: THTPA
• Gene ID (NCBI): 79178
• RRID: AB_2203183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BU02
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The THTPA Polyclonal antibody (Cat# 15486-1-AP) has 1 citation. It was used in a Western blot study published in PLoS One (2014), investigating the metabolic effects of acute thiamine depletion and their reversal by rapamycin in breast and leukemia cells. The research field encompasses thiamine metabolism, cancer biology, and metabolic regulation in human cells.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	PLoS One; Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.; Authors - Shuqian Liu