WB result of THTPA antibody (15486-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THTPA transfected HeLa cells.
WB analysis of K-562 using 15486-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15486-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 15486-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15486-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15486-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15486-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15486-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 15486-1-AP
IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
K-562 cells, A431 cells, HeLa cells, human brain tissue, mouse testis tissue, mouse uterus tissue, PC-3 cells
Positive IP detected in
K-562 cells
Positive IHC detected in
human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
WB result of THTPA antibody (15486-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THTPA transfected HeLa cells.
WB analysis of K-562 using 15486-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15486-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 15486-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15486-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15486-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15486-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15486-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach cancer using 15486-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach cancer using 15486-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of K-562 using 15486-1-AP
IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug.
IF/ICC Figures
IF Staining of HepG2 using 15486-1-AP
Immunofluorescent analysis of HepG2 cells, using THTPA antibody 15486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
THTPA Polyclonal antibody
Catalog Number
15486-1-AP
Citations
1
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
