THTPA Polyclonal antibody, PBS Only

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  WB analysis of K-562 using 15486-1-AP (same clone as 15486-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15486-1-AP (same clone as 15486-1-PBS)

  WB result of THTPA antibody (15486-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THTPA transfected HeLa cells. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 15486-1-AP (same clone as 15486-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 15486-1-AP (same clone as 15486-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse uterus using 15486-1-AP (same clone as 15486-1-PBS)

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 15486-1-AP (same clone as 15486-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 15486-1-AP (same clone as 15486-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15486-1-AP (same clone as 15486-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 15486-1-AP (same clone as 15486-1-PBS)

  IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug. This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15486-1-AP (same clone as 15486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15486-1-AP (same clone as 15486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15486-1-AP (same clone as 15486-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using THTPA antibody 15486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15486-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

THTPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15486-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ThTPase, THTP, Thiamine-triphosphatase, thiamine triphosphatase, EC:3.6.1.28

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Product Information

15486-1-PBS targets THTPA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7834

  Product name: Recombinant human THTPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-230 aa of BC002984

  Sequence: MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG

• Full Name: thiamine triphosphatase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26-35 kDa
• GenBank Accession Number: BC002984
• Gene Symbol: THTPA
• Gene ID (NCBI): 79178
• RRID: AB_2203183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BU02
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.