TIMM44 Polyclonal antibody

TIMM44 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 13859-1-AP

Synonyms

MIMT44, TIM44, TIMM44

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Validation Data Gallery

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  WB analysis using 13859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 13859-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 13859-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13859-1-AP

  IP result of anti-TIMM44 (IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with HeLa cells lysate 1280 ug.

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  IP experiment of mouse heart using 13859-1-AP

  IP result of anti-TIMM44(IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with mouse heart tissue lysate 1680 ug.

• 

  IF Staining of HepG2 using 13859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13859-1-AP (TIMM44 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 13859-1-AP

  Immunofluorescent analysis of A549 cells, using TIMM44 antibody 13859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, C6 cells, Jurkat cells
• Positive IP detected in: HeLa cells, mouse heart tissue
• Positive IF detected in: HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 16 publications below
• IF: See 3 publications below

Product Information

13859-1-AP targets TIMM44 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMM44 fusion protein Ag4834
• Full Name: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Calculated molecular weight: 452 aa, 51 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC033628
• Gene symbol: TIMM44
• Gene ID (NCBI): 10469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Mol Cell; Balancing of mitochondrial translation through METTL8-mediated m3C modification of mitochondrial tRNAs.; Authors - Eva Schöller
human	WB	Nat Commun; Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy; Authors - Jonas Benjamin Michaelis
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.Lines on WB:1. BioRad Precision Plus Protein standards2 and 3. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:13859-1-AP)