TIMM44 Polyclonal antibody

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  WB analysis using 13859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13859-1-AP

  WB result of TIMM44 antibody (13859-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells.

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  WB analysis of Neuro-2a using 13859-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 13859-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13859-1-AP

  IP result of anti-TIMM44 (IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with HeLa cells lysate 1280 ug.

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  IP experiment of mouse heart using 13859-1-AP

  IP result of anti-TIMM44 (IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with mouse heart tissue lysate 1680 ug.

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  IHC staining of human liver using 13859-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13859-1-AP (TIMM44 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (13859-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

TIMM44 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13859-1-AP

Publications(26)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TIMM 44, TIM44, MIMT44

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, C6 cells, Jurkat cells
• Positive IP detected in: HeLa cells, mouse heart tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

13859-1-AP targets TIMM44 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMM44 fusion protein Ag4834
• Full Name: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC033628
• Gene Symbol: TIMM44
• Gene ID (NCBI): 10469
• RRID: AB_2204679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43615
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Mol Cell; Balancing of mitochondrial translation through METTL8-mediated m3C modification of mitochondrial tRNAs.; Authors - Eva Schöller
human	WB	Nat Commun; Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy; Authors - Jonas Benjamin Michaelis
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.Lines on WB:1. BioRad Precision Plus Protein standards2 and 3. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:13859-1-AP)